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Antibacterial Effects of Salvia Officinalis Extract on P.gingivalis & F.nucleatum: An In Vitro Study
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Anahita Ebrahimi   , Vahid Esfahanian * , Arezoo Tahmourespour , Niki Rastghalam |
| Department of Periodontics, Faculty of Dentistry, Isf.c., Islamic Azad University, Isfahan, Iran |
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Abstract: (48 Views) |
Background: The side effects of chemical mouthwashes have led to the increasing use of herbal mouthwashes. Salvia officinalis (S. officinalis) is one of the most valuable medicinal plants from the Lamiaceae family with a broad-spectrum antimicrobial activity. This study aimed to assess the antibacterial effects of S. officinalis against P. gingivalis and F. nucleatum and to compare their susceptibility patterns to the extract using standard in vitro assays.
Materials & Methods: This in vitro Study evaluated the effects of S. officinalis hydroalcoholic extract against standard-strain Porphyromonas gingivalis (P. gingivalis; ATCC3327) and Fusobacterium nucleatum (F. nucleatum; ATCC2558). The antibacterial activity of different concentrations of the extract was evaluated by measuring the diameter of the growth inhibition zones on Mueller-Hinton agar culture medium, and by determining the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the extract in 96-well plates against the bacteria. Data were analyzed by the Kruskal-Wallis and Mann-Whitney U tests (α=0.05).
Results: The mean diameter of the growth inhibition zones caused by 10, 12, and 15 mg/mL concentrations of the extract was significantly larger in the P. gingivalis culture compared with the F. nucleatum culture (P<0.001). Also, the extract at 10, 12, and 15 mg/mL inhibited bacterial growth by 50% (MIC50), 80% (MIC80), and 100% (MBC), respectively.
Conclusion: S. officinalis hydroalcoholic extract showed antibacterial effects against P. gingivalis and F. nucleatum periopathogenic microorganisms. Its MICs were 10 and 12 mg/mL, and its MBC was 15 mg/mL in vitro. |
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Full-Text [PDF 425 kb]
(13 Downloads)
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Type of Study: Research |
Subject:
Periodontics
Received: 2025/10/9 | Accepted: 2026/02/5 | Published: 2026/03/25
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